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KMID : 0380319910480000069
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Journal of Korean Research Institute for Better Living
1991 Volume.48 No. 0 p.69 ~ p.76
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The Effect of Dose of Pregnant Mare´s Serum Gonadotropin on In Vitro Fertilization and Preimplantation Development in Superovulation of Mouse
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Abstract
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A common feature of many in vitro fertilization and embryo transfer(IVF & ET) programs is the stimulation of ovarian follicular development by the administration of exogenous gonadotropins, which routinely results in the recovery of multiple oocytes. However, less favorable consequences of excessive gonadotropin stimulation have been demonstrated in both animal models and human IVF & ET.
The effects of increasing the level of ovarian stimulation on preimplantation embryonic development were assessed using mouse in vitro fertilization system. When F1 hybrid(C57BL×CBA) mice received a single injection of 3IU, 5IU or 10IU PMSG, there was no significant difference in the total number of eggs ovulated.
Increasing the does of PMSG from 3IU to 5IU resulted in significant reduction in the frequency of development to the embryo over 2-cell stage. However, there was no significant reduction by increasing the does of PMSG from 3IU to 10IU.
And no reduction in the frequency of development to the blastocyst or hatching blastocyst was observed when the does of PMSG was increased from 3IU to 5IU or 10IU.
The result suggest that lower does of PMSG(3IU) be favorable to superovulation of mouse to lessen the adverse effect to the oocytes superovulated.
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KEYWORD
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